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PKC-α is phosphorylated by hypertonicity in rat inner medulla. Rat IMCDs were treated with 290-mosmol/kgH2O buffer (iso) or 600-mosmol/kgH2O buffer (hyper) that was made hypertonic by the addition of sucrose. Whole cell lysates were analyzed by Western blot probed for phosphorylated PKC-α (A) or total PKC-α (B). C: bar graph of the ratio of phosphorylated PKC-α to total PKC-α from all samples presented as means ± SE, n = 11/condition. *P < 0.05 vs. Ctrl.
