Fig. 6.

Immunohistochemistry for prosurvival Bcl-2 family proteins Bcl-2 and Bcl-xL. Shown is quantification of Bcl-2- and Bcl-xL-positive cells in tuft corrected with podocyte number in nephritic mice. A: both at baseline and 7 days after disease induction, there were significantly fewer Bcl-2-positive podocytes in double cyclin I^−/− p35^−/− than in wild-type, single cyclin I^−/−, and p35^−/− mice. B: the number of Bcl-xL-positive podocytes was significantly lower in genetically modified mice than in wild-type mice except for single p35^−/− mice. NS, no significant differences. C, control; D, disease (day 7). *P < 0.05 vs. wild-type. **P < 0.05 vs. cyclin I or p35^−/−. Also shown are representative micrographs of immunostaining for Bcl-2 and Bcl-xL. C, G, K, and O: wild-type. D, H, L, and P: cyclin I^−/−. E, I, M, and Q: p35^−/−. F, J, N, and R: cyclin I^−/− p35^−/−. C–F and K–N: unmanipulated. G–J and O–R: glomerulonephritis. Original magnification ×400.