Fig. 1.

Application of NGS for capturing genome-wide associations. The experimental procedures for GCC, Hi-C, biotin-based yeast method, ELP and ChIA-PET are schematically illustrated. All the methodologies start with fixation of in vivo chromatin structures. The 3C-seq method employs 3C, followed by the procedures designed for concentration of small hybrid DNA molecules reflecting genomic associations. In the ChIP-PET method, the fixed samples are immunoprecipitated (ChIP) and the hybrid DNA molecules are enriched by biotin–streptavidin bead purification. The resultant samples are processed by paired-end sequencing.