Effect of chronic occlusion and exercise training on total endothelial NOS (eNOS) and phosphorylated eNOS (p-eNOS, Ser1179) protein levels. Total (A) and phosphorylated (B) eNOS protein levels were significantly increased in coronary arterioles from the collateral-dependent region compared with the nonoccluded region, independent of exercise-training status. Protein was quantified by densitometry analysis, normalized to β-actin, and expressed relative to the density of nonoccluded arterioles of sedentary pigs. Values are means ± SE; n, number of animals. *P ≤ 0.05.