Fig. 8.

Effect of chronic occlusion and exercise training on protein levels of NADPH oxidase subunits. Preliminary evaluation of the Nox family of isoforms revealed that Nox1 and Nox2 were poorly expressed in porcine coronary arterioles (PA), whereas Nox4 was abundant (A). Subsequent evaluation across groups revealed that neither Nox4 (B) nor p47phox (C) was significantly altered by occlusion or exercise training. In contrast, p67phox protein (D) was significantly increased by exercise training, independent of occlusion. Protein was quantified as described in Fig. 3 legend. PL, pig lung; PH, pig heart; PB, pig brain. Values are means ± SE; n, number of animals. *P ≤ 0.05.