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qRT-PCR of selected genes in hearts of wild-type (WT) mice. Values are means ± SE expressed as fold change relative to nonpregnant control group (NP). qRT-PCR was performed in triplicate with a minimum of 4 independent left ventricular samples at different time points. The levels of all candidate genes were normalized to 18S rRNA. See Glossary for definition of abbreviations in A–I. *P < 0.05, significantly different from NP.
