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Effect of PKC activation on Rho kinase activity. Bar graph shows means ± SE values for Rho kinase activity expressed as percentage of control. Basal measurements were compared in cells treated with Y-27632 (10 μM), Stauro (30 nM), and Gen (100 μM). Effect of PMA (500 nM) was measured in control cells, as well as in cells pretreated with Y-27632, Stauro, or Gen; n = 3 for each experiment. *Significant difference from control value; †significant difference from PMA.
