Fig. 5.
Regulation of endogenous adenosine tone. A: the equilibrative nucleoside transporter inhibitors S-(4-nitrobenzyl)-6-thioinosine (NBTI; 5 μM) + dipyridamole (DIPY; 10 μM) reduce the amplitude of evEPSCs in BF cholinergic neurons, and this effect is blocked in the presence of DPCPX (500 nM). B: effect of the adenosine kinase inhibitor 5-iodotubercidin (5-IT; 7 μM) on evEPSC amplitude. C: effect of the adenosine deaminase inhibitor erythro-9-(2-hydroxy-3-nonyl)adenine hydrochloride (EHNA; 10 μM) on evEPSC amplitude. All evEPSCs were recorded at Vh = −70 mV. Time courses of the effects of NBTI + DIPY, 5-IT, and EHNA applied in control ACSF (●) and in the presence of DPCPX (○) are shown in graphs at right.