Figure 1.

The P-element insertion in Pcf11 intron 1 causes alternative splicing. (A) The cDNA was prepared from strain l(2) K08015/+; the 5′-end sequencing result is partly shown. The double-peaked graph can be read in two sequences. (B) The BLAST search results indicate that the two sequences match exons 2 and 5. Some exons in RD are numbered on the shaded boxes (open reading frame with brown color). The vertical bars show the matching regions. The green arrowhead below the blue bar representing the Pcf11 gene denotes the position of the P insertion. The other three arrows above the bar denote the positions of the other mutations analyzed in this study.