Figure 2.

Lesions identified in the Pcf11 gene from the Inr-a mutations. (A) A stop codon found in the Inr-a^EMS-2/+ heterozygote. A base pair of C:G was changed to T:A by EMS mutagenesis, thus changing 456R to a stop codon (Z). The double peaks indicate a nucleotide change. DNA sequence and its translation are shown. (B) A replacement of 7 bp with 5 bp (colored in DNA sequences) was found in the Inr-a^γC/+ heterozygote. A series of double peaks is shown. The original and new DNA sequences and the translation of the original one are indicated. (C). Double peaks were found in Pcf11 intron 4 in the Inr-a^hd1/+ heterozygote. This change is in the conserved splicing donor site (shaded). DNA sequences of the exon intron were shown as upper- and lowercased letters.