. 2012 Jun 1;2(6):707–720. doi: 10.1534/g3.112.001990

Table 1. Primers used for gene expression analysis by RT-PCR.

Target Gene	Primer Set (5′→3′)
ghr	Forward: CCCACTGCCCCCTGTATCT
ghr	Reverse: CTTCAGAAGGAGGCTGTTTTGC
β-actin	Forward: GCTGTCTTCCCCTCCATCGT
β-actin	Reverse: TCTCCCACGTAGCTGTCTTTCTG
igf1	Forward: CAGGCATCCAGATTGTGCAA
igf1	Reverse: ACCATGTTCTGAGAATTCCTGTGTT
igf1r	Forward: AGACCCAGTTTCTGAATTTCACC
igf1r	Reverse: GTTCTTATAAGGCGCCTCTTTGT
ef1	Forward: GCCCCTCCAGGATGTCTACA
ef1	Reverse: ACGGCCCACGGGTACTG
18s	Forward: CCCCGTAATTGGAATGAGTACACTTT
18s	Reverse: ACGCTATTGGAGCTGGAATTACC

For details, see Materials and Methods. RT-PCR, reverse transcription polymerase chain reaction; ghr, growth hormone receptor; β-actin, beta actin; igf1, insulin growth factor-1; igf1r, insulin growth factor-1 receptor; ef1, elongation factor-1; 18s, 18s ribosomal subunit.