Fig. 2.
Pharmacological profiling of H. contortus levamisole-sensitive receptors reveals a selective sensitivity of Hco-L-AChR1 subtype to bephenium. Two-microelectrode voltage-clamp recording traces of inward currents elicited by a series of cholinergic agonists in X. laevis oocytes (Ach acetylcholine, Pyr pyrantel, Nic nicotine, Beph bephenium and Lev levamisole). Horizontal bars indicate when agonists are applied. All oocytes were treated with 100 μM BAPTA-AM for 4 h prior to recording. All recordings were performed with 1 mM external CaCl2. a (Left panel) Hco-L-AChR1 reconstituted by coinjection of an equimolar amount of 7 cRNAs (Hco-unc-29.1, Hco-unc-38, Hco-unc-63, Hco-acr-8, Hco-ric-3.1, Hco-unc-50, Hco-unc-74) displayed inward currents elicited by the 5 cholinergic agonist drugs. (Right panel) Schematic representation of the Hco-L-AChR1 possible subunit composition. The subunit in white can be any of the 4 others. b (Left panel) Hco-L-AChR2 reconstituted by coinjection of an equimolar amount of 6 cRNAs (Hco-unc-29.1, Hco-unc-38, Hco-unc-63, Hco-ric-3.1, Hco-unc-50, Hco-unc-74) omitting Hco-acr-8 is not activated by bephenium. (Right panel) Schematic representation of the Hco-L-AChR2 possible subunit composition. The subunits in white can be any of the 3 others