Fig. 6.

Evaluation of the potency of selective kinase inhibitors. HEK293 cells were transiently transfected with CLuc-FGF14 and CD4-Nav1.6-NLuc and treated with the indicated concentrations of PD169316 (A) or BAY 11-7082 (B). Dose–response inhibition of the FGF14:Nav1.6 channel C-tail assembly upon treatment with PD169316 (four replicates from one experiment) and BAY 11-7082 (eight replicates from two independent experiments); data are mean±SEM. The IC₅₀ calculated by fitting the nonlinear regression equation (dotted line) is 8.9±0.93 μM (PD169316) and 8.8±0.85 μM (BAY 11-7082).