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Evaluation of assay reliability. Luminescence values from HEK293 cells transiently transfected with CLuc-FGF14+CD4-Nav1.6-NLuc and either treated with 0.5% DMSO (positive control) or BAY 11-7082 (negative control and/or background) were 7970±150 RLU and 84.2±4.5 RLU, respectively. Signal-to-noise ratio was 142, signal-to-background was 95.3, and signal window was 5.3. Data are mean±SD; the statistical parameters were calculated over n=32 wells for each group.
