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. 2012 Jan 16;3(4):885–892. doi: 10.3892/ol.2012.568

Figure 2.

Figure 2

Effects of VEGF siRNA-V2 on the viability and apoptotic state of Tca8113 cells. (A) VEGF siRNA-V2 inhibited Tca8113 cell viability as assayed with a cell counting kit using WST-8 dye. Results are shown as the mean absorbance ± SEM of three independent experiments. (B) VEGF siRNA-V2 promoted Tca8113 cell apoptosis. The cells were detected by Annexin V/PI double staining, and representative dot plots of Annexin-V/PI staining are shown: lower left quadrant, viable (double negative) population; lower right quadrant, apoptotic (AnnexinV+/PI) population; upper left quadrant, necrosis (Annexin V-/PI+) population; upper right quadrant, late apoptotic dead cells (Annexin V+/PI+). (C) The percentages of Annexin V positive cells are shown. Data are shown as the means ± SEM of three independent experiments. *P<0.05 versus control. VEGF, vascular endothelial growth factor; siRNA, small interfering RNA; PI, propidium iodide.