Table I.
Primer and probe sequences for the four SNPs.
| SNP | Primer sequence | Probe sequence | |
|---|---|---|---|
| hMLH1 | |||
| -93G>A (rs1800734) | Forward Reverse |
5′-GCCAATCACCTCAGTGCCTC-3′ 5′-TGAGAAATTTGACTGGCATTCAAG-3′ |
A 5′F-TCTTCCTTTAGCTGTAGCT-P3′ G 5′H-TCTTCCTTCAGCTGTAG-P3′ |
| I219V (rs1799977) | Forward Reverse |
5′-CACTACCCAATGCCTCAACC-3′ 5′-TGAAAGGTTCCAAAATAATGTGA-3′ |
A 5′F-TCGCTCCTTCTTT-P3′ G 5′H-TCGCTCCCTCTTT-P3′ |
| hMSH2 | |||
| -118T>C (rs2303425) | Forward Reverse |
5′-TGCTTATGATTGGTTGCCG-3′ 5′-CCTGGTTGAAGAAAATGCG-3′ |
T 5′F-ACACCCAATCAGCTT-P3′ C 5′H-ACACCCAGTCAGCTT-P3′ |
| IVS12-6T>C (rs2303428) | Forward Reverse |
5′-GAATATATGTTGATTTACCTCCCATATTG-3′ 5′-CAATCCATTTATTAGTAGCAGAAAGAAGTT-3′ |
T 5′F-CCTACAAAACAAATTA-P3′ C 5′H-CCTACAGAACAAATTA-P3′ |
SNP, single nucleotide polymorphisms; F, FAM; H, HEX; P, TaqMan-MGB.