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Methylation analysis of the DAPK gene in CML blast crisis patients. Examples are provided for patients 3–8 in the M primer. Patient 5 shows methylation in the DAPK promoter. Patients 3, 4 and 6–8 show no methylation in the DAPK promoter. P, positive control (universally methylated DNA); N, negative control (peripheral blood DNA from a healthy individual); H, H2O blank (PCR negative control); M, 100 bp DNA ladder.
