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Representative RFLP analysis of the SmaI digest of the PCR product that contains position 276 of the ADIPOQ gene separated on a 2% agarose gel. Lane M: DNA marker. Lane 1: uncut PCR product of size 372 bp. Lane 2: Homozygous (GG) shows two bands of sizes 219 and 153 bp. Lane 3: Heterozygous (TG) shows three bands of sizes 372, 219 and 153 bp. Lane 4: Wild-type (TT) shows one band of size 372 bp. RFLP, restriction fragment length polymorphism; PCR, polymerase chain reaction.
