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. 2012 Jun;139(6):493–505. doi: 10.1085/jgp.201110685

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© 2012 Frederiksen et al.

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Figure 1. — 11-cis 4-OH retinal is more soluble in aqueous buffer than 11-cis-retinal. (A and B) Plots of measured concentration of 11-cis 4-OH retinal (filled circles) and 11-cis-retinal (open triangles) against nominal retinoid concentration in Ringer’s solution. Values of measured concentration were estimated from spectral absorbance using an extinction coefficient (ε = 20,800 M⁻¹ cm⁻¹) obtained by fitting a straight line to the linear range (three first data points) of the absorbance measurements of 11-cis 4-OH retinal (inset in A). Data in A were measured on a solution that contained no BSA. The data in B were measured on a solution that contained 0.01% BSA. Continuous lines connect data points for 11-cis 4-OH retinal; dashed lines show 11-cis-retinal data points. The inset in B shows stick diagrams of the two species of retinal. Error bars represent means ± SD.