Table 3.
Kinetics of H2O2 emission from mitochondria under FET in the absence or presence of inhibitors
| Condition | Mouse | Rat | Guinea pig | ||||
| State 4 | State 3 | State 4 | State 3 | State 4 | State 3 | ||
| H2O2 emissiona | Control | 107 ± 1.5 | 82 ± 1.5 | 105 ± 2.5 | 80 ± 5 | 95 ± 3 | 62 ± 3 |
| +AF | 707 ± 65 | 585 ± 49 | 440 ± 29 | 271 ± 30 | 791 ± 92 | 678 ± 91 | |
| +DNCB | 2085 ± 195 | 767 ± 165 | 2541 ± 210 | 1309 ± 144 | 512 ± 47 | 558 ± 31 | |
| K0.5 | AF (nM) | 1.32 ± 0.5 | 0.4 ± 0.2 | 12.5 ± 3.7 | 15 ± 7 | 3.5 ± 1.5 | 4.1 ± 1.9 |
| DNCB (µM) | 0.95 ± 0.27 | 0.8 ± 0.5 | 2.1 ± 0.4 | 2.0 ± 0.76 | 1.05 ± 0.16 | 1.7 ± 0.12 | |
a
H2O2 emission in all cases is expressed in pmol min−1 mg−1 protein.