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. 2012 May 29;7(5):e38010. doi: 10.1371/journal.pone.0038010

Figure 1. Localization of the Drosophila Rad9, Hus1 and Rad1 proteins in S2R+ and follicle cells.

Figure 1

A–G, Confocal images of S2R+ cells, I–K, Confocal images of follicle cells from egg chambers. (A) S2R+ cells expressi ng HA-DmHus1 and stained with anti-HA antibodies in red. (B) S2R+ cells expressing GFP-DmRad1. (C) S2R+ cells expressing GFP-DmRad9A. (F) S2R+ cells expressing DmRad9B-GFP. (D) and (G) Staining with anti-lamin antibodies, which mark the nuclear membrane, in red. (E and H) are merged image of (C with differential interference contrast (DIC) image) and (F with a DIC image), respectively. (I) Egg chamber from HA-DmHus1::CY2Gal4 transgenic flies. (J) Egg chamber from GFP-DmRad1::CY2Gal4 transgenic flies. (K) Egg chamber from FLAG-DmRad9A::CY2Gal4 transgenic flies. In both S2R+ and follicle cells, DmHus1 is found in the cytoplasm, DmRad1 is found throughout the cell and Dm DmRad9A is localized to the nuclear membrane. DmRad9B is localized to the nucleus in S2R+ cells.