Table 1. SK-N-SH cell death after OGD depends on RelA-Lys310 acetylation.
Reative LDH release | ||
C | OGD15h | |
pSG5 | 0,05±0,005 | 0,27±0,02 |
RelA | 0,04±0,003 | 0,45±0,04* |
RelA-K310R | 0,06±0,002 | 0,13±0,01# |
RelA-K310Q | 0,04±0,003 | 0,4 0±0,04* |
Data show the cell survival of SK-N-SH cells over-expressing a control vector, pSG5, or wild-type RelA or RelA-K310R or RelA-K310Q constructs and exposed to 15 hours of OGD, compared to controls. Wild-type RelA and RelA-K310Q over-expression significantly enhanced toxicity due to OGD, while RelA-K310R over-expression prevented cell loss. Data are mean±s.e.m of three separate experiments run in triplicate and represent the amount of LDH released by each well, relative to total releasable LDH.
p<0,0005 RelA or RelA-K310Q OGD vs relative pSG5;
p<0,0005 RelA-K310R OGD vs relative RelA.