. 2012 May 29;7(5):e37939. doi: 10.1371/journal.pone.0037939

Table 1. Binding parameters for poly(U)•poly(A)_*poly(U) triplex-alkaloid complexation obtained from McGhee-von Hippel analysis of the Scatchard plot from the spectrophotometric and fluorimetric titration data at 25°C^a.

Alkaloid	Absorbance		Fluorescence
	K_i /10⁵ M⁻¹	n	K_i /10⁵M⁻¹	n
BC	2.88±0.02	4.3	3.9±0.05	4.1
BC1	8.65±0.34	4.2	9.5±0.10	4.0
BC2	39.87±0.41	4.3	47.0±0.56	4.3

Average of four determinations in each case. K_i is the intrinsic binding constant to an isolated binding site and ‘n’ represents the number of excluded sites.

Table 1. Binding parameters for poly(U)•poly(A)*poly(U) triplex-alkaloid complexation obtained from McGhee-von Hippel analysis of the Scatchard plot from the spectrophotometric and fluorimetric titration data at 25°Ca.

Table 1. Binding parameters for poly(U)•poly(A)_*poly(U) triplex-alkaloid complexation obtained from McGhee-von Hippel analysis of the Scatchard plot from the spectrophotometric and fluorimetric titration data at 25°C^a.