Table 3. ITC Derived Thermodynamic parameters for the binding of BC, BC1 and BC2 with poly(U)_•poly(A)_*poly(U) triplex at 25°C.^a .

Parameters	BC	BC1	BC2
Ka  /105 m −1	1.5±0.04	9.73±0.09	23±0.10
N	4.7	4.5	4.5
Δ G o (kcal mol −1 )	−7.10±0.02	−8.22±0.04	−8.73±0.05
Δ H o (kcal mol −1 )	−4.86±0.03	−2.30±0.05	−1.65±0.05
T Δ S o (kcal mol −1 )	2.24	5.92	7.08

^aAll the data in this table are derived from ITC experiments and are average of four determinations. K_a and ΔH ^o values were determined from ITC profiles fitting to Origin 7 software as described in the text. n is the site size which is the reciprocal of the stoichiometry N. The values of ΔG ^o and TΔS ^o were determined using the equations ΔG ^o  =  − RTln K_a and ΔG ^o  = ΔH ^o − TΔS ^o. All the ITC profiles were fit to a model of single binding site.