Table 1. Statistics for glial, neuronal, SSR areas and ratios.
| Category | NMJn = | Larvaen = | Glia: MeanArea +/− S.D. | α-HRP: MeanArea +/− S.D. | SSR: MeanArea +/− S.D. | Glia/HRP | Glia/SSR |
| WT F3 18°C | 24 | 13 | 11.71+/−2.93 | 22.30+/−4.94 | 20.27+/−4.72 | 0.59+/−0.14 | |
| WT F3 25°C | 28 | 23 | 11.80+/−3.75 | 20.97+/−3.59 | 18.78+/−3.92 | 0.63+/−0.17 | |
| WT F3 30°C | 28 | 19 | 15.46+/−4.06 | 24.42+/−6.10 | 22.49+/−6.10 | 0.71+/−0.11 | |
| Fixed F3 25°C | 12 | 12 | 7.30+/−3.66 | 18.95+/−4.77 | 0.39+/−0.183 | ||
| Fixed W3 25°C | 14 | 14 | 9.11+/−2.79 | 18.27+/−3.44 | 0.50+/−0.135 | ||
| hiw 18°C | 24 | 13 | 14.95+/−4.40 | 27.03+/−5.33 | 0.55+/−0.12 | ||
| hiw 25°C | 21 | 11 | 17.58+/−4.31 | 29.50+/−4.83 | 0.60+/−0.13 | ||
| hiw 30°C | 28 | 21 | 17.10+/−5.37 | 27.12+/−4.75 | 0.65+/−0.24 | ||
| TTX 30°C | 18 | 9 | 10.44+/−3.67 | 20.39+/−3.25 | 0.51+/−0.14 | ||
| WT 3018°C | 29 | 18 | 13.01+/−4.01 | 23.35+/−4.28 | 0.56+/−0.14 | ||
| WT 1830°C | 18 | 12 | 11.11+/−2.66 | 21.10+/−3.76 | 0.53+/−0.12 | ||
| W3 25 °C T0′ | 20 | 12 | 13.82+/−3.18 | 21.01+/−3.92 | 0.66+/−0.13 | ||
| W3 25°C T60′ | 19 | 12 | 13.79+/−3.36 | 21.13+/−4.41 | 0.66+/−0.12 |
Mean and standard deviation (SD) values for measured areas of glial processes (CD8-GFP), neurons (anti-HRP immunolabeling) and SSR (ShCter-DsRed). Glial processes were measured from the terminal region of axon as it enters the synapse. The ratios of glial process area to neurons or SSR are indicated. Replicate numbers of NMJs and larvae are indicated for each experimental protocol. F3, feeding third instar larvae. W3, wandering third instar larvae. WT, wild-type. Fixed, fixed larvae; all other larvae were live. T0, T60; 0 and 60 minute time points. 30→18°C, temperature down-shift from 30 to 18°C after hatching. 18→30°C, temperature up-shift from 18 to 30°C after hatching. Areas are in µm2.