Figure 3. Efficient Treg depletion for all infection groups.

CD4⁺CD25^hi T cells were isolated by magnetic bead separation. Treg frequencies were defined as percentages of CD25^hiFOXP3⁺ cells from total CD4⁺ fractions for mock- and CD4⁺CD25^hi cell -depleted PBMC that were cultured for 4 days in medium (representative example in A). Treg frequency of mock (M) and depleted (D) PBMC is shown for all donors (B) as well as for the different infection groups (C). Lines represent data points from one individual, data were analyzed by non-parametric paired tests; ***p≤0.001 ****p≤0.0001 or p-value as indicated.