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. 2012 May 29;7(5):e37761. doi: 10.1371/journal.pone.0037761

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Tostar et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The transfected SUFU and/or GLI1FL constructs were detected using Myc and FLAG antibodies by confocal microscopy. A, Individual transfections of Myc-tagged SUFU-FL or SUFU-ΔC and FLAG-tagged GLI1FL constructs. B, Co-transfections of GLI1FL with the SUFU-FL (FL) or the SUFU-ΔC (ΔC) construct. Two representative images from each co-transfection are shown. The nuclei are stained with the marker DRAQ5 (blue signal). Note that GLI1FL co-localizes with SUFU-ΔC in aggregate/clump structures in the cytoplasm.