Figure 5. Nanomechanical analysis of Sup35NM.

ΔL _c (left) and F (right) histograms of pFS-2 polyproteins carrying Sup35NM. Like the wt proteins in Figures 3 and 4, Sup35NM (first row) shows a broad polymorphism, including rare hM conformers. Treatment with QBP1 peptide (20 µM [42], back row) decreases the formation of M and hM conformers, as seen for both polyQ and α-synuclein (Q₆₂ and A53T, Figures 2 and 4). TEM images of the amyloid fibers formed by I27+Sup35NM are shown on the right. Addition of QBP1 clearly reduces the formation of amyloid fibers. The scale bars correspond to 0.6 (top) and 0.4 µm (bottom).