Fig. 2. Repression of CRM activity bv ectopic En and Eve.

Map at top: CRMs named in bold italics drive a 14-stripe slp1-like pattern (Fujioka and Jaynes, 2012), adjacent to En stripes. The red and blue filled boxes indicate CRMs where we identified functional En binding sites, as shown in later figures.

All rows: embryos carried the transgenic CRM-lacZ reporter indicated across the top.

2^nd and 4^th rows: embryos also carried a heat shock-inducible transgene driving ubiquitous En (2^nd row) or Eve (4^th row) expression. Embryos were heat shocked and stained as described in Materials and Methods. Note in the 2^nd row that expression driven by each CRM except u4739 is significantly repressed by En, and that all of the CRMs in the 4^th row are repressed by Eve, u2316 less completely so than the others, as indicated in Fig. 1.