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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Cell Signal. 2012 Mar 30;24(8):1496–1503. doi: 10.1016/j.cellsig.2012.03.017

Figure 1. mAKAP and MEF2 expression show a parallel increase during myogenic differentiation.

Figure 1

(A) C2C12 myoblasts were washed with PBS extensively before the addition of differentiation media. Cell lysate was collected at the time points indicated and proteins were analyzed by immunoblot. n=3. (B) Quantification of the western blots shown in A, normalizing the actin. n=3. (C) C2C12 myoblasts were infected with an adenoviral MEF2 luciferase reporter and β-galactosidase. The resulting transcriptional activity was read at the times indicated. Normalized luciferase values were obtained by dividing the luciferase value by the β-galactosidase control value. n=3. Data shown are mean SEM from three representative experiments.