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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Cell Signal. 2012 Mar 30;24(8):1496–1503. doi: 10.1016/j.cellsig.2012.03.017

Figure 2. mAKAP binds MEF2 in proliferating and differentiating C2C12 cells.

Figure 2

(A) MEF2 was immunoprecipitated from C2C12 myoblast cell lysates using an antibody that recognizes all the known isoforms of the transcription factor. mAKAP binding was demonstrated by immunoblot. n=3 (B) The mAKAP complex was immunoprecipitated from C2C12 myoblast cells using an antibody specific for the AKAP. MEF2 association was determined using an antibody that recognizes all isoforms of MEF2. n=3. (C &D) MEF2 was immunoprecipitated during a short (C) and long (D) time course of C2C12 differentiation using an antibody that recognizes all isoforms of MEF2. Differentiation of C2C12 cells was induced by incubating the cells in differentiation media for the indicated period of time. MEF2 was immunoprecipitated and mAKAP association was determined by immunoblot. (n=3).