Skip to main content
. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Cell Signal. 2012 Mar 30;24(8):1496–1503. doi: 10.1016/j.cellsig.2012.03.017

Figure 4. mAKAP binds the first 91 amino acids of MEF2.

Figure 4

(A) Schematic diagram depicting the MEF2 expression fragments used for this study. (B) The MEF2 binding fragment of mAKAP (301-510) was expressed as a GST fusion protein. Beads charged with either the mAKAP fragment or control protein were incubated with HEK293 cell lysates transfected with either GFP-tagged full length MEF2 (B), amino acids 1-91 of MEF2 (C), or amino acids 91-410 (D). Association was visualized by immunoblot using an antibody that recognized the GFP tag on the fragments. Total protein expression is shown in the middle bands while a protein stain of the GST fragments used is shown in the lower panel. n=3. (E) MEF2A was immunoprecipitated from C2C12 cells and association of mAKAP was demonstrated by western blot. (F)