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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Nat Immunol. 2012 Apr 22;13(6):579–586. doi: 10.1038/ni.2282

Figure 2. The Fam49b gene is the source of a new antigen presented by Qa-1b in the absence of ERAAP function.

Figure 2

(a) cDNA pools were transfected into Qa-1b-expressing L cells, ERAAP function inhibited and BEko8Z hybridoma responses measured. One cDNA pool in plate 23 contained the antigenic activity. (b) A schematic of the clone 23D9.15 showing the Fam49b gene and the locations of the PCR primers used to make truncations. Truncations take the name of the reverse PCR primer used. The BEko8Z stimulatory activity was in region 13 (R13). The locations of PCR primers used to establish the C-terminus of the final peptide are shown on the amino acid sequence of R13. The indicated minigenes were tested as described for panel a. Data is graphed as Mean±SEM, of transfections done in duplicate. One representative of two experiments is shown.