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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Reprod Toxicol. 2012 Mar 5;33(4):563–576. doi: 10.1016/j.reprotox.2012.02.004

Fig. 6. PFOA treatment reduces mammary gland protein levels of Areg, HGF, ERα, EGFR and PCNA in Balb/c and C57Bl/6 wild type mice but not in C57Bl/6 PPARα knockout mice.

Fig. 6

A. Effect of PFOA treatment on mammary gland protein levels of Areg, HGF, ERα, EGFR and PCNA. Balb/c, C57Bl/6 wild type and PPARα knockout mice were sacrificed 24 h after the last vehicle control or PFOA treatment. Protein samples were prepared from frozen mammary glands and used for Western blot analysis as described in Materials and Methods. Representative Western blots of protein levels of Areg, HGF, ERα, EGFR, PCNA and PPARα in vehicle control- or PFOA-treated mammary glands. GAPDH served as a protein loading control. B. Quantification of Western blot analysis for the effect of PFOA treatment on mammary gland protein levels of Areg, HGF, ERα, EGFR and PCNA in Balb/c and C57Bl/6 wild type and PPARα knockout mice (mean ± standard deviation, n=3). * p< 0.05, compared with Vehicle Control-treated group in each mouse strain.