Table 3.
Supplement of exogenous estradiol (E), progesterone (P) or E+P overcomes PFOA mammary gland inhibition in Balb/c and C57Bl/6 wild type mice (mean ± standard deviation, n=5)
Treatment | Ductal length (relative units) | Number of TEBs | AMGW (g) | RMGW (%) |
---|---|---|---|---|
Balb/c | ||||
Vehicle Control | 3.24 ± 1.32 | 5.40 ± 2.61 | 0.096±0.018 | 0.55±0.09 |
PFOA (2.5mg/kg)+7dC | 1.28 ± 1.33a | 1.67 ± 1.03a | 0.110±0.019 | 0.64±0.08 |
PFOA (2.5mg/kg)+7dE | 2.76 ± 0.57b | 9.40 ± 1.95a,b | 0.098±0.019 | 0.55±0.06 |
PFOA (2.5mg/kg)+7dP | 3.13 ± 0.94b | 5.20 ± 1.79b | 0.094±0.024 | 0.56±0.14 |
PFOA (2.5mg/kg)+7d(E+P) | 2.90 ± 0.53b | 7.40 ± 2.07b | 0.094±0.013 | 0.56±0.09 |
C57Bl/6 Wild type | ||||
Vehicle Control | 2.60 ± 0.53 | 4.60 ± 2.51 | 0.106±0.011 | 0.66±0.07 |
PFOA (7.5mg/kg)+7dC | 0.72 ± 0.80a | 0.67 ± 0.82a | 0.110±0.020 | 0.77±0.14 |
PFOA (7.5mg/kg)+7dE | 2.53 ± 0.45b | 7.00 ± 1.41b | 0.116±0.026 | 0.72±0.17 |
PFOA (7.5mg/kg)+7dP | 1.60 ± 0.46 | 2.67 ± 0.58b | 0.116±0.015 | 0.75±0.12 |
PFOA (7.5mg/kg)+7d(E+P) | 2.13 ± 023b | 7.20 ± 1.10b | 0.118±0.011 | 0.73±0.06 |
Three-week old female Balb/c or C57Bl/6 wild type mice were treated with vehicle control or PFOA for 1 week, then the mice were treated with vehicle control or PFOA plus C (vehicle), E, P or E+P for another week as described in Materials and Methods. Mice were sacrificed 24 h after the last PFOA/hormone treatment and mammary gland whole mounts were prepared as described in Materials and Methods.
Relative mammary glands weight (RMGW) (%) = absolute mammary glands weight (AMGW)/BW
p< 0.05, compared with Vehicle Control-treated group in each mouse strain.
p< 0.05, compared with PFOA-treated group + 7dC in each mouse strain.