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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Reprod Toxicol. 2012 Mar 5;33(4):563–576. doi: 10.1016/j.reprotox.2012.02.004

Table 3.

Supplement of exogenous estradiol (E), progesterone (P) or E+P overcomes PFOA mammary gland inhibition in Balb/c and C57Bl/6 wild type mice (mean ± standard deviation, n=5)

Treatment Ductal length (relative units) Number of TEBs AMGW (g) RMGW (%)
Balb/c
 Vehicle Control 3.24 ± 1.32 5.40 ± 2.61 0.096±0.018 0.55±0.09
 PFOA (2.5mg/kg)+7dC 1.28 ± 1.33a 1.67 ± 1.03a 0.110±0.019 0.64±0.08
 PFOA (2.5mg/kg)+7dE 2.76 ± 0.57b 9.40 ± 1.95a,b 0.098±0.019 0.55±0.06
 PFOA (2.5mg/kg)+7dP 3.13 ± 0.94b 5.20 ± 1.79b 0.094±0.024 0.56±0.14
 PFOA (2.5mg/kg)+7d(E+P) 2.90 ± 0.53b 7.40 ± 2.07b 0.094±0.013 0.56±0.09
C57Bl/6 Wild type
 Vehicle Control 2.60 ± 0.53 4.60 ± 2.51 0.106±0.011 0.66±0.07
 PFOA (7.5mg/kg)+7dC 0.72 ± 0.80a 0.67 ± 0.82a 0.110±0.020 0.77±0.14
 PFOA (7.5mg/kg)+7dE 2.53 ± 0.45b 7.00 ± 1.41b 0.116±0.026 0.72±0.17
 PFOA (7.5mg/kg)+7dP 1.60 ± 0.46 2.67 ± 0.58b 0.116±0.015 0.75±0.12
 PFOA (7.5mg/kg)+7d(E+P) 2.13 ± 023b 7.20 ± 1.10b 0.118±0.011 0.73±0.06

Three-week old female Balb/c or C57Bl/6 wild type mice were treated with vehicle control or PFOA for 1 week, then the mice were treated with vehicle control or PFOA plus C (vehicle), E, P or E+P for another week as described in Materials and Methods. Mice were sacrificed 24 h after the last PFOA/hormone treatment and mammary gland whole mounts were prepared as described in Materials and Methods.

Relative mammary glands weight (RMGW) (%) = absolute mammary glands weight (AMGW)/BW

a

p< 0.05, compared with Vehicle Control-treated group in each mouse strain.

b

p< 0.05, compared with PFOA-treated group + 7dC in each mouse strain.