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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Integr Biol (Camb). 2012 May 3;4(6):661–671. doi: 10.1039/c2ib20009k

Fig. 3.

Fig. 3

Chromatin accessibility is correlated with RelA induction threshold. (A) Heterochromatin fraction was quantified with a DNAse I sensitivity assay. Quantitative PCR was performed in triplicate and normalized to a hemoglobin-β (HBB) reference gene. (B–C) Correlation of heterochromatin fraction with (B) induction threshold and (C) activation coefficient extracted from the fits in Fig. 2D–F. (D–F) Chromatin immunoprecipitation for (D) total H3, (E) H3K9me3 and (F) acetylated H3 bound to the HIV promoter in unstimulated clones was correlated to the induction threshold. Quantitative PCR was performed in triplicate and normalized to an input control. Data are presented as the mean ± standard deviation. Differences are labeled as significant (*) if p < 0.05. Pearson correlation coefficient R is indicated on plot.