Fig. 2.

RayBio Human Cytokine Antibody Array of pooled culture supernatants from PBMC from ten adult patients with dcSSc stimulated for 6 days with 10 μg/ml native bovine CI. After 6 days of incubation of PBMC plus 10 μg/ml CI, culture supernatants were pooled and analyzed, along with the culture medium alone (complete DMEM) as a control, in a RayBio Cytokine and Growth Factor Antibody Array assay. The assays used contained antibodies to 120 cytokines, and receptors and 41 growth factors impregnated in small circles on membranes along with positive and negative controls. All antibodies are bound in small duplicate circles on the membranes. Drawn circles indicate cytokines that were enhanced above the background. Unstimulated PBMC produce small amounts of cytokines spontaneously thereby preventing their use as controls