Fig. 4.

SSc dermal fibroblasts cultured for 14 days with pro-inflammatory cytokines followed by stimulation with TNF-α for 24 h. Inhibition of constitutively produced MMP-1 as well as TNF-α-stimulated MMP-1 production was observed when SSc 008 fibroblasts were cultured with PDGF-BB and IL-13 when compared to SSc fibroblasts cultured with the medium alone for 14 days ± TNF-α. MMP-1 was increased when fibroblasts were cultured for 14 days with PDGF-AA or EGF and then stimulated 24 h with TNF-α. *P < 0.05 when compared to supernatants from fibroblasts cultured with the complete medium alone (first bar on the left). ^# P < 0.05 when compared to supernatants from fibroblasts cultured with the complete medium plus TNF (second bar from the left)