Figure 3.

Relationship of Ft-Ds-Dachs and Core Protein Polarization

(A–E) Images of EGFP-Dachs expressed in patches under the actin promoter in the wing disc near to the dorsal hinge in WT (F) and fz^P21 mutant background (G) labeled for GFP (green) and Arm (magenta).

(B–E) Shown are magnifications of specific regions of the disc as marked in (A). Arrows show the direction of Fz-EYFP asymmetry pointing distally (toward the center of the wing pouch). Numbers refer to strength of Fz-EYFP asymmetry in individual clones as a ratio of fluorescence levels on cell boundaries on distal edges of clones versus proximal edges. There is a high degree of variation in the strength of asymmetry between clones, with an average asymmetry of 6.0 ± 0.96 (SEM).

(F and G) Images of EGFP-Dachs expressed in patches under the actin promoter in the wing disc near to the dorsal hinge in WT (F) and fz^P21 mutant background (G) labeled for GFP (green) and Arm (magenta). Arrowheads point to distal cell junctions where EGFP-Dachs is enriched and arrows point to proximal junctions with less Dachs. Asymmetry of EGFP-Dachs was measured and no significant difference detected between WT and fz mutant (ratio of distal to proximal EGFP-Dachs WT = 2.9 ± 0.35 [SEM] and fz^P21 = 2.5 ± 0.21 [SEM], >40 clones).

(H–L) Images of Fz-EYFP expressed in patches under the actin promoter in wing discs in a dachs^GC13/dachs¹ mutant background labeled for GFP (green) and Arm (magenta).

(I–L) Shown are magnifications of specific regions of the disc as marked in (H). In dachs^GC13/dachs¹ discs, Fz-EYFP predominately points distally except in dorsal clones close to the hinge and distant from the AP boundary (L) where the direction of Fz-EYFP asymmetry follows the pouch-hinge boundary. This was not the case in WT (E). In (I)–(K), numbers refer to strength of Fz-EYFP asymmetry in individual clones as ratio of distal/proximal fluorescence levels. In (L), the ratio of Fz-EYFP fluorescence relative to the axis of the pouch-hinge boundary is indicated: PD asymmetry is negligible in these clones (PD ratios are 1.2 and 0.7).

(M) Mapped orientation of Fz-EYFP asymmetry in the dorsal half of wing discs in WT, dachs^GC13/dachs¹ and ft^G-rv dachs^GC13/ ft⁸ dachs¹. Red arrows indicate Fz-EYFP clones in which asymmetry was misoriented (pointing away from the AP boundary).

See also Figure S3.