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. 2012 Mar 22;43(1):22. doi: 10.1186/1297-9716-43-22

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Copyright ©2012 Verbrugghe et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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The effect of T-2 toxin on the cell viability. Percentage viability (%) of Salmonella Typhimurium infected and uninfected (A) PAM exposed to different concentrations of T-2 toxin (0.250-10 ng/mL), (B) undifferentiated IPEC-J2 cells exposed to different concentrations of T-2 toxin (0.500-10 ng/mL), (C) differentiated IPEC-J2 cells exposed to different concentrations of T-2 toxin (0.500-100 ng/mL). Twenty-four hours after incubation with T-2 toxin, the cytotoxic effect was determined by neutral red assay. Results represent the means of 3 independent experiments conducted in triplicate and their standard deviation. Superscript (*) refers to a significant difference compared to the control group (p < 0.05).