Table 3.
Differential protein expression of differentiated IPEC-J2 cells after exposure to T-2 toxin.
| Protein name* | Function* | Protein ratio treated/untreated IPEC-J2 cells on the T-test approach | Protein ratio treated/untreated IPEC-J2 cells on the log*log approach |
|---|---|---|---|
| Cytochrome c oxidase subunit VIIc {N-terminal} | This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. | 0.6 | 0.6 |
| Microsomal glutathione S-transferase 3 | Functions as a glutathione peroxidase. | 0.6 | 0.6 |
| PREDICTED: similar to Keratin, type I cytoskeletal 18 (Cytokeratin 18) | When phosphorylated, plays a role in filament reorganization. | 0.7 | 0.7 |
| Myristoylated alanine-rich C-kinase substrate | Myristoylated alanine-rich C-kinase substrate is a filamentous (F) actin cross-linking protein. | 0.7 | 0.7 |
| Annexin A4 | Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. | 0.7 | 0.7 |
| Chain A, Bovine Mitochondrial F1-Atpase Complexed With Aurovertin B | Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane. | 0.7 | 0.8 |
| Protein S100-A16 | Calcium-binding protein. Binds one calcium ion per monomer. | 0.8 | 0.8 |
| Putative beta-actin | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. | 0.8 | 0.7 |
| Cysteine and glycine-rich protein 1 isoform 1 | Encodes a member of the cysteine-rich protein (CSRP) family that includes a group of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. | 1.2 | 1.2 |
| Heat shock protein 60 | Implicated in mitochondrial protein import and macromolecular assembly. | 1.2 | 1.2 |
| PREDICTED: similar to nucleolin-related protein isoform 3 | Plays a role in different steps in ribosome biogenesis. | 1.2 | 1.2 |
| Heterogeneous nuclear ribonucleoprotein F | Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. | 1.2 | 1.2 |
| Heat shock protein 10 | Essential for mitochondrial protein biogenesis, together with chaperonin 60. | 1.2 | 1.2 |
| Thymosin beta-10 | Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. | 1.2 | 1.2 |
| Thioredoxin-related transmembrane protein 1 | May participate in various redox reactions. | 1.3 | 1.3 |
| Glutathione S-transferase P | Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. | 1.3 | 1.3 |
| 14-3-3 protein sigma | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signalling pathway. of G2/M progression. | 1.3 | 1.3 |
| Elongation factor 1-beta | Elongation factor 1-beta and Elongation factor 1-delta stimulate the exchange of GDP bound to Elongation factor 1-alpha to GTP. | 1.3 | 1.3 |
| Profilin | Binds to actin and affects the structure of the cytoskeleton. | 1.5 | 1.5 |
| Cyclophilin A or Peptidyl-prolyl cis-trans isomerase A | Peptidyl-prolyl isomerase accelerates the folding of proteins. | 1.6 | 1.6 |
| Branched-chain-amino-acid aminotransferase, cytosolic | Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. | 1.6 | 1.6 |