Fig. 3.

mRNA ontogenic expression of part of the Cyp2 family (Cyp2c66, Cyp2c67, Cyp2c68, Cyp2c69, Cyp2c70, Cyp2d9, Cyp2d10, Cyp2d11, Cyp2d12, Cyp2d22, Cyp2d26, Cyp2d34, Cyp2d40, Cyp2e1, Cyp2f2, Cyp2g1, Cyp2j5, Cyp2j6, Cyp2j7, and Cyp2j8) in male and female livers from day −2 to 45 days of age. Total RNA was isolated from livers at each age (n = 5 per gender at each age). Gender was not differentiated from day −2 to 5 days of age (n = 5 at each age). The mRNA expression levels for Cyp2c67, Cyp2c68, Cyp2c69, Cyp2d9, Cyp2d10, Cyp2d11, and Cyp2d12 were determined with RT-PCR assays, whereas the mRNA levels for other P450s were quantified with the multiplex branched DNA assay, as described under Materials and Methods. Data are presented as the fluorescence intensity of mRNA (mean ± S.E.M.) normalized to Gapdh levels. *, statistically significant differences between male and female (p < 0.05).