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. 2012 Jun;40(6):1226–1237. doi: 10.1124/dmd.111.042697

Fig. 4.

Fig. 4.

mRNA ontogenic expression of part of the Cyp2 family (Cyp2j9, Cyp2j11, Cyp2j13, Cyp2j13, Cyp2t4, Cyp2u1, and Cyp2w1), the Cyp3 family (Cyp3a11, Cyp2a13, Cyp2a16, Cyp3a25, Cyp3a59, Cyp3a41a, Cyp3a41b, and Cyp3a44), and part of the Cyp4 family (Cyp4a10, Cyp4a12a/b, Cyp4a14, Cyp4a29, and Cyp4a30b) in male and female livers from day −2 to 45 days of age. Total RNA was isolated from livers at each age (n = 5 per gender at each age). Gender was not differentiated from day −2 to day 5 of age (n = 5 at each age). The mRNA expression levels for Cyp2j12, Cyp3a59, Cyp3a41b, and Cyp4a12a/b were determined with RT-PCR assays, whereas the mRNA levels for other P450s were quantified with the multiplex branched DNA assay, as described under Materials and Methods. Data are presented as the fluorescence intensity of mRNA (mean ± S.E.M.) normalized to Gapdh levels. *, statistically significant differences between male and female (p < 0.05).