TABLE 1.

Primer sequences for validation of novel transcripts identified by RNA-Seq

Gene	Method	Amplification	Direction	Sequence (5′ to 3′)	PCR Product
					bp
Cyp2b10	Endpoint PCR	From exon 6 to exon 9 (including cassette exon)	Forward	ATGGCTTCCTGCTCATGCTCAAGT	411
			Reverse	GACAAATGCGCTTTCCCACAGACT
	Sequencing	From exon 7 to exon 9		AGTGCCACACAGAGTGACCAAAGA
Cyp2d26	Endpoint PCR	From exon 5 to cassette exon	Forward	ACTACACATCCCTGGTTTGCCTGA	520
			Reverse	AGCCTCTGAGCACCTTCTCTTGTA
	Sequencing	From exon 6 to cassette exon		TGATTGACCTGTTCATGGCAGGGA
	Endpoint PCR	From cassette exon to exon 9	Forward	TACAAGAGAAGGTGCTCAGAGGCT	327
			Reverse	TAGGGCTCTGGAGTAACTGGCATT
	Sequencing	From exon 8 to cassette exon		CATGAAGGCCTCGTGCTTCACAAA
Cyp3a13	3′-RACE	cDNA synthesis		GCGAGCACAGAATTAATACGACTCACTATAGGT12VN
		Outer PCR	Forward	AAGTTGCTCTTGTCAGAGTCCTGC
			Reverse	GCGAGCACAGAATTAATACGACT
		Inner PCR	Forward	CACTGTCCAGCCTTGTAAGGAAAC
			Reverse	CGCGGATCCGAATTAATACGACTCACTATAGG
		Sequencing		AAGTTGCTCTTGTCAGAGTCCTGC