Increased oxidative stress in the inferior colliculus of Sepp1−/− mice. A, Images of the inferior colliculus from WT Sepp1+/+ (top row) and Sepp1−/− (bottom row) mice probed with an antibody for 8-oxo-dG and visualized with DAB staining. Higher magnification images (right) B, Normalized optical density measurements for 8-oxo-dG immunoreactivity in the inferior colliculus. *p<0.05. C, Confocal images (20x) showing PV (left), 8-oxo-dG (middle), and colocalization of the markers (right). Scale bar: 200 μm.