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. 2012 May 30;6:77. doi: 10.3389/fnins.2012.00077

Figure 4.

Figure 4

Ethanol-impaired neurogenesis is mediated by IL-1β. HEC slices at 10 DIV were treated with ethanol for 96 h in the presence or absence of IL-1β (50 ng/ml), neutralizing antibodies against IL-1β (E + Anti-IL-1β, 2 μg/ml), TNFα (E + Anti-TNFα, 2 μg/ml), and MCP-1 (E + Anti-MCP-1, 5 μg/ml) or IL-1 surface receptor antagonist (E + IL-1RIa, 2 μg/ml). Bar graph (A) shows mean ± SEM of DCX IR measured from each group. Ethanol-impaired neurogenesis is exacerbated by exogenous IL-1β and reversed by addition of neutralizing anti-IL-1β antibody or blockade of IL-1 surface receptors. ANOVA F(6, 38) = 11.27, p < 0.0001, and Fisher post hoc: ***p < 0.001 compared with corresponding group as indicated, n = 6–8. Representative DCX+ IR photographs from the groups as indicated were shown in (B–E). The experiments were repeated at least four times with similar designs and results (scale bar = 200 μm).