Figure 9.

Rolipram and anti-oxidant BHT protect from ethanol-impaired neurogenesis. Shown in bar graph (A): PDE IV inhibitor rolipram and anti-oxidant BHT increase DCR+ IR and reverse ethanol inhibition of DCX+ IR. ANOVA F(5, 32) = 11.552, p < 0.001, and Fisher post hoc: ***p < 0.001 or **p < 0.01 compared with Control or EtOH4D, n = 6–8. The experiments were repeated with similar results. Representative photographs of DCX+ IR are shown in (B) (control); (C) (EtOH); (D) (Ethanol + BHT, 50 mM, pretreatment for 3 days); and (E) (Ethanol + Rolipram, 500 nM, pretreatment for 3 days; scale bar = 200 μm).