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. 2012 Jun;341(3):725–734. doi: 10.1124/jpet.111.191247

Fig. 6.

Fig. 6.

Cisplatin-mediated renal inflammatory gene expression is attenuated by inhibition of soluble epoxide hydrolase. Mice were treated daily with 100 mg/kg AR9273 or vehicle orally for 5 days, and a single dose of 20 mg/kg cisplatin was administered on day 2. Kidneys and blood were harvested 24 to 72 h after cisplatin treatment and used for measurement of inflammatory markers. A and B, renal TNFα (A) and ICAM-1 (B) mRNA levels were measured by quantitative real-time PCR. RNA levels were normalized to Gapdh, and the data are presented relative to the vehicle-treated group. C and D, renal TNFα (C) and serum ICAM-1 (D) protein levels were quantified by EIA. The values shown are the mean ± S.D. of 8 to 10 mice per treatment group. Significant differences are indicated: *, p < 0.05, **, p < 0.01, and ***, p < 0.001 between vehicle- and cisplatin-treated mice; †, p < 0.05, ††, p < 0.01, and †††, p < 0.001 between mice treated with cisplatin alone and cisplatin with AR9273.