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. 2012 Jun;81(6):778–787. doi: 10.1124/mol.111.076828

Fig. 6.

Fig. 6.

RAD51 and Polζ are necessary for resolving platinating agent-induced DSBs. REV3- and Polη-depleted HeLa cells were treated with cisplatin (5 μM), oxaliplatin (15 μM), satraplatin (2 μM), and picoplatin (25 μM) for 1 h; washed; and then fixed 24 and 48 h later. Cells were stained for S1981P-ATM and53BP1 as surrogate markers of DNA DSBs. Nuclear DNA was stained with 4,6-diamidino-2-phenylindole (DAPI). A, the percentage of cells exhibiting 10 or more colocalized foci containing both S1981P-ATM and 53BP1 was determined. Data represent the mean ± S.E.M. from four independent experiments where >100 cells were counted in each experiment. B, the difference in percentage of cells displaying ≥10 colocalized foci containing both S1981P-ATM and 53BP1 between 24 and 48 h is shown. C, representative images of control-, REV3- and Polη-depleted cells treated with satraplatin are shown. The presence or absence of Polη had little impact on the efficiency of ICL-induced DSB resolution.