Fig. 3.

Effects of PCNA-I on PCNA association with chromatin. PC-3 cells were treated with 1 μM PCNA-I1 for various times (A) or for 8 h with various concentrations of PCNA-I1 (B). The NP-E and NP-R fractions of protein were analyzed by immunoblotting using PC10 PCNA antibody or using an antibody to histone 1 (chromatin-associated protein control) or α-tubulin (NP-40 extractable free form PCNA control). C, MCF-7, HeLa, or LNCaP cells were treated for 8 h with 1 μM PCNA-I1. The NP-E and NP-R fractions were analyzed as described for PC-3 cells in A and B. Data shown are from one experiment that is representative of three.